How fast does RNA polymerase II transcribe our genes?
New paper from Gregersen lab is online now.
Liu and Gregersen developed DRB/TTchem-seq2, a powerful enhancement of existing methods that enables direct measurement of RNA polymerase II (RNAPII) elongation rates across more than 3,000 genes.
This approach combines rapid timepoint sampling with a new computational framework to track RNAPII movement with unprecedented resolution.
Key takeaways:
• DRB/TTchem-seq2 increases individual gene elongation rate measurements by approximately 7-fold.
• The computational pipeline can be independently applied to existing DRB/TTchem-seq datasets.
• Elongation rates vary widely both between and within genes.
• Specific histone modifications and elongation factor occupancy correlate with distinct elongation profiles.
Kinetic measurement of gene-specific RNA polymerase II transcription elongation rates